
Shenandoah CTGrade重组蛋白系列是来自大肠杆菌的重组人蛋白,旨在支持基础、转化、临床研究和商业化应用。
特点
Shenandoah CTGrade重组蛋白系列是来自大肠杆菌的重组人蛋白,旨在支持基础、转化、临床研究和商业化应用。
特点
通知
The three proteins, LC3, GABARAP (GABAA receptor-associated protein), and GATE-16 (Golgi-associated ATPase enhancer), are mammalian homologues of yeast Atg8. Among them, LC3 has been studied most extensively and frequently used as an autophagy marker in mammals. Newly translated LC3 (proLC3) is immediately processed at the C-terminus by Atg4B or Atg4A, forming LC3-I. Upon induction of autophagy, LC3-I is sequentially transferred to E1 and E2, and conjugated to the substrate, PE (phosphatidylethanolamine). The resulting PE-conjugated LC3 is called LC3-II. Although LC3-II has a higher molecular weight than LC3-I, the mobility of LC3-II is greater than LC3-I on SDS-PAGE, due to higher hydrophobicity. GABARAP and GATE-16 are also conjugated to PE in a similar process.