Products

  • Large Stokes shift
  • For use with simultaneous multicolor imaging
  • For use with single laser line FCCS
Detection of mitophagy with Keima-Red

CoralHue™ dimeric Keima-Red (dKeima-Red) and CoralHue™ monomeric Keima-Red (mKeima-Red) are red fluorescent proteins with extremely large Stokes shift. They absorb light maximally at 440 nm and emit red light at 616 nm and 620 nm, respect ively. There are no other fluorescent proteins with this unique fluorescence. Because of this characteristic, they are excited by a very short wavelength but emit a long wavelength. Keima is named after a shogi (Japanese chess) piece Keima (桂馬) that can move in the hopping manner, similar to the knight in the game of chess.

The large Stokes shift property of Keima-Red allows effective applications such as for single wavelength excitation simultaneous multi-color imaging and single laser line FCCS.

FEATURE

  • Serum Free
  • Stabilized Wnt3a
  • High Activity



Organoid culture

Wnt signaling is known to be involved in early development, maintenance and regeneration of stem cells, and in cancer formation. Wnt signaling has also been found to play an important role in the growth and maintenance of these processes. In particular, Wnt3a has been revealed to be an essential niche component for maintaining the proliferation of Lgr5-positive stem cells in intestinal epithelial cells and is used for the production of various digestive organoids such as the small intestine, large intestine, stomach, pancreas and liver. Although Wnt3a has been conventionally used for the culture of gut organoids, it is a fat-soluble protein, so it forms aggregates in serum-free medium and can not exert its activity sufficiently. In 2016, Mihara et al. found that high Wnt3a activity can be maintained by forming a complex with Wnt3a by Afamin, which is one of the components of serum. In addition, by using Afamin and Wnt3a complex for organoid culture, long-term culture of organoid becomes possible. This new medium will result in optimal success for your organoid experiments.

 

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