Wako Anti Iba1, Monoclonal Antibody (NCNP24)
Iba1 is a protein highly expressed in microglia and macrophage with a molecular weight of about 17 kDa. The protein is a commonly known as a microglial marker in the central nervous system.
Recently, microglia has been extensively studied because they are responsible for immune function in the central nervous system, and are related to various diseases such as neurodegenerative diseases, mental illuness, brain tumors and infection.
- 1. LINK
- 2. Features
- 3. Applications: Immunohistostaining
- 4. Antibody information
- 5. Product List
- 6. Related Products
1. LINK
- Leaflet
(1,066KB/2p) - Anti Iba1, Rabbit, Polyclonal antibodies ⇒ LINK
- Labeled Iba1 Antibodies ⇒ LINK
2. Features
- Evaluated by immunohistochemistry
- Useful for multicolor immunostaining
- Monoclonal antibody with high specificity and low background
3. Applications – Immunohistostaining –
→ Clear localization signals of microglia using Anti Iba1, Monoclonal Antibody (Wako Cat. No.012-26723)
as well as using Anti Iba1, Rabbit polyclonal antibody (Wako Cat. No.019-19741 019-19741).
| ♦ Assay Condition:
· Sample: cerebral cortex of 7-week Wistar rat, 7-week ICR mouse, or adult marmoset · Section: 50μm-frozen section (mouse and rat), 40μm-frozen section (marmoset) · Staining method: ABC method + DAB staining · Antibody concentration = 1: 500 |
All immunohistochemistry data were provided by Sanagi,T., Manabe,T., Ichinohe, N., and Kohsaka, S., National Center of Neurology and Psychiatry in Japan.
4. Antibody information
| ANTI IBA1, MONOCLONAL ANTIBODY (NCNP24) | |
|---|---|
| ANTIGEN | Synthetic peptide (C-terminal of Iba1) |
| CLONE NO. | NCNP24 |
| SUBCLASS | Mouse IgG1 |
| CONCENTRATION | 1.3 mg/mL (the first lot) |
| BUFFER | 50% Glycerol / TBS, 0.05% Sodium Azide |
| SPECIES REACTIVITY | Mouse, Rat, Marmoset |
| APPLICATION | Immunohistochemistry (frozen section) 1:500 – 2,000) |
5. Product List
| PRODUCT NAME | PKG. SIZE | WAKO CAT. NO. | STORAGE CONDITION |
|---|---|---|---|
| Anti Iba1, Monoclonal Antibody (NCNP24) | 10 μL | 012-26723 | Keep at -20°C. |
| 50 μL | 016-26721 |
6. Related Products
| PRODUCT NAME | PKG. SIZE | WAKO CAT. NO. | STORAGE CONDITION |
|---|---|---|---|
| Anti Iba1, Rabbit (for Immunocytochemistry) ⇒ details | 50 μg | 019-19741 | Keep at -20°C. |
| Anti Iba1, Rabbit (for Wstern Blotting) ⇒ details | 50 μg | 016-20001 | |
| Anti Iba1, Rabbit, Biotin-conjugated ⇒ details | 100 μL | 016-26461 | Keep at 2-10°C. |
| Anti Iba1, Rabbit, Red Fluorochrome (635)-conjugated ⇒ details | 100 μL | 013-26471 |
| PRODUCT NAME | PKG. SIZE | WAKO CAT. NO. | STORAGE CONDITION |
|---|---|---|---|
| Anti Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64) | 50 μg | 015-25191 | Keep at -20°C. |
| Anti Phophorylated α-Synuclein, Monoclonal Antibody (pSyn#64), Biotin-conjugated ⇒ details |
100 μL | 010-26481 | Keep at 2-10°C. |
| Anti Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64), FITC-conjugated ⇒ details |
100 μL | 017-26491 |