NS Basal Medium is a chemically-defined synthetic medium optimized for culturing nerve cells. NS Supplement is a serum-free supplement (containing retinyl acetate) specifically designed for supporting the growth of nerve cells. When mixed together, they can be used for culturing a variety of nerve cells, including rat hippocampal neurons and neural stem cells.
Primary Culture of Rat Hippocampal Neurons
Rat hippocampal neurons isolated from E19 embryos were cultured using poly-L-lysine-coated microtiter plates.
The morphology of these neurons was analyzed on day 6. We also examined the expression of the neuronal markers MAP2(a+b) and the glial marker GFAP on day 21
Day 6
< Growth Medium >
NS Basal Medium containing 2% NS Supplement and 0.5 mM L-glutamine
< Seeding Density >
13,000cells/well（96well plate）
Day 21
Primary Culture of Rat Cortical Neurons
Primary cortical neurons isolated from E17 rat embryos were cultured using poly-L-lysine-coated microtiter plates.
The morphology of these neurons was analyzed on day 8. We also examined the expression of the neuronal markers MAP2(a+b) and the glial marker GFAP on day 14.
Day 8
< Growth Medium >
NS Basal Medium containing 2% NS Supplement and 0.5 mM L-glutamine
< Seeding Density >
60,000cells/well（96well plate）
Day 14
