N2 supplement is an alternative to serum for culturing neural stem cells. It is suited for culturing primary neurons and neural stem cells. Differentiation of neural stem cells is induced by the components of FBS. Thus, serum alternatives like N2 supplement are used in order to maintain undifferentiated states of the cells. There are two products in this series: one prepared with apo-transferrin, and another prepared with holo-transferrin.

N2 Supplement with Transferrin (apo)

N2 supplement containing iron-free apo-transferrin.

Since the amount of ferrous ion to be added in the culture medium is minimized, it may be suitable for culturing cells that are sensitive to oxidative stress.

N2 Supplement with Transferrin (Holo)

Regular composition.

Comparing the Number of Neurons Derived from Rat Hippocampus

 

＜Culture medium＞
D-MEM/Ham’s F-12 + 2mmol/l L-glutamine + 1xN2 Supplement
+ 1xpenicillin-streptomycin + 25ng/ml bFGF

＜Number of cells seeded＞
16,000cells/cm2（12-well plate）

＜Culture condition＞
　 37℃, 5%CO₂

Culturing Neural Stem Cells Derived from Rat Hippocampus

N2 Supplement with Transferrin (Holo)

 

N2 Supplement with Transferrin (apo)

Product List

N2 Supplement with Transferrin(Apo)(×100)

for Cell Culture

Storage Condition : 

Keep at -20 degrees C.

N2 Supplement with Transferrin(Holo)(×100)

for Cell Culture

 

Wako Anti Iba1, Monoclonal Antibody (NCNP24)

Iba1 is a protein highly expressed in microglia and macrophage with a molecular weight of about 17 kDa. The protein is a commonly known as a microglial marker in the central nervous system.
Recently, microglia has been extensively studied because they are responsible for immune function in the central nervous system, and are related to various diseases such as neurodegenerative diseases, mental illuness, brain tumors and infection.

• 1. LINK
• 2. Features
• 3. Applications: Immunohistostaining
• 4. Antibody information
• 5. Product List
• 6. Related Products

1. LINK

• Leaflet  (1,066KB/2p)
• Anti Iba1, Rabbit, Polyclonal antibodies ⇒ LINK
• Labeled Iba1 Antibodies ⇒ LINK

2. Features

• Evaluated by immunohistochemistry
• Useful for multicolor immunostaining
• Monoclonal antibody with high specificity and low background

3. Applications – Immunohistostaining –

                        → Clear localization signals of microglia using Anti Iba1, Monoclonal Antibody (Wako Cat. No.012-26723)
as well as using Anti Iba1, Rabbit polyclonal antibody (Wako Cat. No.019-19741 019-19741).

All immunohistochemistry data were provided by Sanagi,T., Manabe,T., Ichinohe, N., and Kohsaka, S., National Center of Neurology and Psychiatry in Japan.

4. Antibody information

5. Product List

6. Related Products