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KESSEL, Sarah, et al. (2016). High-Throughput 3D Tumor Spheroid Screening Method for Cancer Drug Discovery Using Celigo Image Cytometry. Journal of laboratory automation: 2211068216652846.
http://jla.sagepub.com/content/early/2016/06/03/2211068216652846.abstract
Glioblastoma: U87MG, high-throughput screening by Image cytometry, Spheroid and Cell Viability Spheroid Size and Adherent Cell Confluence Measurement, Fluorescent Staining Celigo Image Cytometer, 3D tumor spheroid, spheroid size, spheroid viability, glioblastoma, high-throughput screening, image cytometry, Celigo, 17-AAG paclitaxel TMZ doxorubicin(DOX), MS-9384U
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OZAWA, Masayuki (2015). The N-cadherin cytoplasmic domain confers anchorage-independent growth and the loss of contact inhibition. Scientific Reports. 5: 1-16.
http://www.nature.com/articles/srep15368
Madin-Darby canine kidney cell: MDCK T23 cells, anchorage independence growth of tumor cells, Immunoprecipitation and immunoblotting Flow cytometry Anoikis assay Fluorescence microscopy WST-1 cell pr liferation assay Soft agar colony assay Gene expression microarray and data analysis, Phase contrast micrograph Fluorescence microscopy, anchorage independence growth of tumor cells E-cadherin N-cadherin YAP/TAZ signaling, Doxorubicin (DOX), PrimeSurface
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YONESAKA, Kimio, et al. (2015). The pan-HER family tyrosine kinase inhibitor afatinib overcomes HER3 ligand heregulin-mediated resistance to EGFR inhibitors non-small cell lung cancer. Oncotarget. 6: 33602.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741788/pdf/oncotarget-06-33602.pdf
Human lung adenocarcinoma: PC9Mock, heregulin-overexpressing PC9HRG cells, Cell viability assay, Annexin V binding assay Cell-proliferation inhibition assays(CellTiter-Glo Luminescent Cell Viability Assay (Promega, Madison, WI, USA).) Xenograft studie, CellTiter-Glo, heregulin non-small cell lung cancer human epidermal growth factor receptor, Afatinib Erlotinib, MS-9060X, MS-9096U
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TARIQ, Mohammad, et al. (2016). Eukaryotic translation initiation factor 5A (eIF5A) is essential for HIF-1α activation in hypoxia. Biochemical and Biophysical Research Communications. 470: 417-424.
Cervical cancer cell line: HeLa, A549, RNA-interference Quantitative PCR Luciferase Assay Immunoblotting, Celigo Imaging Cytometer (Nexcelom Bioscience), Acetylation eIF5A HIF-1α Hypoxia Hypusination, MG132 GC7, MS-9096U
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TERASHIMA, Jun, et al. (2016). VEGF expression is regulated by HIF‐1α and ARNT in 3D KYSE‐70, esophageal cancer cell spheroids. Cell biology international.
http://onlinelibrary.wiley.com/doi/10.1002/cbin.10656/abstract;jsessionid=2D6393AC8CD7651247E6CF4979307310.f01t04
Esophageal carcinoma cell: KYSE‐70,, RNAi assay, Western blotting, 3D culture; 3D spheroid; hypoxia-inducible factor 1α(HIF-1α) vascular epithelial growth factor(VEGF), HIF-1alpha RNAi, MS-9096U
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MASUDA, Mari, et al. (2016). TNIK inhibition abrogates colorectal cancer stemness. Nature communications. 7: 12586.
Hhuman embryonic kidney cell line : HEK293 Human colorectal cancer cell line: DLD-1 Human colorectal cancer cell line: HCT116 Patient-derived cancer-initiating spheroids, Cancer stem cells, Real-time RT–PCR Tumour multiplicity. Mobility shift assay Kinase selectivity profiling Luciferase reporter assay Immunoblot analysis Imunohistochemistry Drug sensitivity assay Soft-agar colony-formation assay Cell cycle analysis Crystallization X-ray data collection, structure determination, and refinement. ALDH activity, SDS–PAGE DABMap detection kit and Mobility shift assay： QuickScout Screening Assist Kit (07-138MS, Carna Biosciences)., Cancer stem cells Colon cancer TNIK inhibition abrogates Colorectal cancer stemness, NCB-0846 NCB-0970 (NCB-0005 & KY-05009), MS-9096U MS-9060X or MS-9035X
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SUDA, Yoshitaka, et al. (2016). Clonal heterogeneity in osteogenic potential of lung cancer-associated fibroblasts: promotional effect of osteogenic progenitor cells on cancer cell migration. Journal of cancer research and clinical oncology: 1-12.
http://link.springer.com/article/10.1007%2Fs00432-016-2171-y
Human lung adenocarcinoma epithelial cell line: A549 Cancer-associated fibroblasts (CAFs), cancer cell migration generation of single-cell- derived CAFs clones, cell migration, A plate scanner, Nano Zoomer (Hamamatsu Photonics, Japan), Mesenchymal progenitor cell Cancer-associated fibroblast (CAF) Lung cancer Tumor microenvironment Osteogenic potential Single-cell-derived clones, siRNA, MS-9096U
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EETEZADI, Sina, et al. (2015). Effects of Doxorubicin Delivery Systems and Mild Hyperthermia on Tissue Penetration in 3D Cell Culture Models of Ovarian Cancer Residual Disease. Molecular pharmaceutics. 12: 3973-3985.
http://pubs.acs.org/doi/10.1021/acs.molpharmaceut.5b00426
Ovarian cancer cell lines: HEYA8, OV-90, and SKOV3., Cytotoxicity, drug penetration, and efficacy analysis in tumor spheroid block copolymer micelles-DDS, Imaging cell toxicity, Confocal microscopy, doxorubicin, Doxil, Caelyx, nanomedicines, block copolymer micelles, ovarian cancer, multicellular tumor spheroids, hyperthermia, Doxorubicin (DOX), MS-9096U
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MISU, Masayasu, et al. Effects of Wnt-10b on proliferation and differentiation of murine melanoma cells. Biochemical and Biophysical Research Communications, 2015, 463. 4: 618-623.
http://ac.els-cdn.com/S0006291X15300371/1-s2.0-S0006291X15300371-main.pdf?_tid=84a4189e-464d-11e5-987d-00000aacb35e&acdnat=1439973625_d5580e59a9e1638ebbbfc53efeed6ed5
Melanoma cells: B16, Melanoma, SA-b-gal staining Scratch assay TOPFLASH assay Transplantation Cell proliferation analysis, Melanoma Wnt signal Wnt-10b Differentiation, MS-9096U
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NAKANISHI, Yoshito, et al. Mechanism of oncogenic signal activation by the novel fusion kinase FGFR3–BAIAP2L1. Molecular cancer therapeutics, 2015, 14. 3: 704-712.
http://mct.aacrjournals.org/content/14/3/704.full.pdf
Rat-2 fibroblast cell: Rat-2_F3-B-DBAR Cell, sphenoid formation assay, MS-9096U
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NOZAWA-SUZUKI, Noriko, et al. The inhibitory effect of hypoxic cytotoxin on the expansion of cancer stem cells in ovarian cancer. Biochemical and Biophysical Research Communications, 2015, 457. 4: 706-711.
http://ac.els-cdn.com/S0006291X15000832/1-s2.0-S0006291X15000832-main.pdf?_tid=7417027a-464d-11e5-b9d2-00000aab0f6c&acdnat=1439973597_ed000437f350908f78e13a3d156658ca
Ovarian cancer cell lines: SKOV3, SKOV3ip1, Ovarian cancer, 2.2. Western blotting: HIF-1a, HIF-2a, b-actin 2.3. Real time PCR 2.6. Immunofluorescenc staining, Light microscopy, Ovarian Cancer Hypoxia HIF (hypoxia inducible factor); HIF-1a and HIF-2a Cancer stem cell Stem cell factors: Oct4, Nanog, Sox2 and Lin28 CD133, CD44, TX-402, an analogue of tirapazamine, a hypoxia selective prodrug with inhibitory effects of HIF-1 and angiogenesis, MS-9096U
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ROTEM, Asaf, et al. Alternative to the soft-agar assay that permits high-throughput drug and genetic screens for cellular transformation. Proceedings of the National Academy of Sciences, 2015, 112. 18 5708-5713.
http://www.pnas.org/content/early/2015/04/15/1505979112.abstract
Ovarian cancer cells derived from patients with chemotherapy-resistant disease(Bevacizumab, Carboplatin,Gemcitabine,Iniparib, Paclitaxel, Pazopanib,Rucaparib, Sapacitabine,Topocetan, Selicilib), Breast cancer cell line: MCF-10A, MDA-MB-231, MDA-MB-468, T47D Prostate cancer cell Ovarian cancer cell line:SAA1, SAA2, SAA3 Prostate cancel cell line: BPH1, PC3 Fibroblast cell line: EH, EL, ELR, WI-38, Evaluation of drug efficacy of vaariable drugs against patient derived primary cancer cells, which have drug restistance due to multiple drug therapy., Soft-Agar Assays.(Colony formation in soft agar) ATP Assay Flow Cytometry (Calcein-AM, CD45-FITC, EPCAM-PE, and CD24-PE/Cy),, ATP assay PCR(Illumina sequencing), Transformation Drug screen Genetic screen Personalized medicine (Patient Derived ) PrimeSurface384U Colony formation in soft agar ATP Assay Oncogenes (H-Ras, K-Ras, and EGFR), Azelastine hydrochloride Nitazoxanide RepSox Nobiletin, MS-9384UZ
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YONESAKA, K, et al. Anti-HER3 monoclonal antibody patritumab sensitizes refractory non-small cell lung cancer to the epidermal growth factor receptor inhibitor erlotinib. Oncogene, 2015
http://www.nature.com/onc/journal/vaop/ncurrent/full/onc2015142a.html
Non-small cell lung cancer, Non-small cell lung cancer, Cell proliferation and growth inhibition assay, Cell Titer Glo, Non-small cell lung cancer Anti-HER3 monoclonal antibody Epidermal growth factor receptor(EGFR) inhibitor, Patritumab Erlotinib, MS-9060X
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YOSHIDA, Ryohei, et al. EGFR tyrosine kinase inhibitors combined with cytotoxic drugs for treatment of NSCLC with EGFR gene mutations: Efficacy and mechanisms. Cancer research, 2015, 75. 15 Supplement: 3501-3501.
http://cancerres.aacrjournals.org/content/75/15_Supplement/3501.short
Lung cancer cell lines :PC9, PC9GR, HCC827, HCC827GR, and H1975, Lung cancer, EGFR - Tyrosine Kinase Inhibitor Cytotoxic drug, Docetaxel Gefitinib AZD9291, MS-9096U
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KODAMA, Tatsushi, et al. A Novel Mechanism of EML4-ALK Rearrangement Mediated by Chromothripsis in a Patient-Derived Cell Line. Journal of Thoracic Oncology, 2014
JFCR-LC649, an EML4-ALK-positive cell line established from pleomorphic carcinoma, a rae subtype of non-small-cell lung cancer (NSCLC) NCI-H2228, NCI-H522, Non–small-cell lung cancer, Immunohistochemistry Cell Growth Inhibition and Caspase-3/7 Assay Immunoblotting and Immunoprecipitation, Fluorescence was quanti ed using Envision (PerkinElmer, Waltham, MA)., ALK (Anaplastic large cell lymhoma) inhibitor Chromosomal rearrangement Chromothripsis, Alectinib, MS-9096U
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KODAMA, Tatsushi, et al. Alectinib shows potent antitumor activity against RET-rearranged non–small cell lung cancer. Molecular cancer therapeutics, 2014, 13. 12: 2910-2918.
http://mct.aacrjournals.org/content/13/12/2910.long
Non–small cell lung cancer cell: LC-2/ad cells, NCI-H2228 cells, Ba/F3, Non-small cell lung cancer, Kinase inhibitory assay Cell growth inhibition assay Caspase-3/7 assay qRT-PCR, Non-small cell lung cancer, Alectinib Crizotinib Cabozantinib Vandetanib Gefitinib LDK378, MS-9096U
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MIKHAIL, Andrew S., et al. Image-based analysis of the size-and time-dependent penetration of polymeric micelles in multicellular tumor spheroids and tumor xenografts. International journal of pharmaceutics, 2014, 464.1: 168-177.
http://www.sciencedirect.com/science/article/pii/S0378517314000209
Human cervical cancer cells line: HeLa Human colon cancer cell line: HT29, Nanomedicine, Light microscopy, Nanoparticle Block copolymer micelle Spheroid Tumor penetration Nanomedicine Tumor Xenografts, MS-9096U
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MORI, Masamichi, et al. The Selective Anaplastic Lymphoma Receptor Tyrosine Kinase Inhibitor ASP3026 Induces Tumor Regression and Prolongs Survival in Non–Small Cell Lung Cancer Model Mice. Molecular cancer therapeutics, 2014, 13.2: 329-340.
http://mct.aacrjournals.org/content/13/2/329.long
Mouse non-small cell lung cancer model NCI-H228, Non-small cell lung cancer, antiproliferative assay, Cell Titer Glo, Survival in non-small cell lung cancer Model mice Tyrosine kinase inhibitor, Receptor Tyrosine Kinase Inhibitor ASP3026, MS-9096U
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OHNISHI, Ken, et al. Plastic induction of CD133AC133-positive cells in the microenvironment of glioblastoma spheroids. International Journal of Oncology, 2014, 45.2: 581-586.
http://www.spandidos-publications.com/ijo/45/2/581
Human glioblastoma cell: T98G, Glioblastoma spheroids Cancer stem cell, Frozen cryostat sectionsImmuno staining:CD133AC133 /HIF-1α/DAPI Real-time quantitative PCR: CD133, Immunofluorescent staining, cancer stem cell, CD133, glioblastoma, spheroid Hypoxia, MS-9035X MS-9096U
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SHIMOZATO, O., et al. Receptor-type protein tyrosine phosphatase κ directly dephosphorylates CD133 and regulates downstream AKT activation. Oncogene (Nature), 2014, 1-12.
http://dx.doi.org/10.1038/onc.2014.141
Colon adenocarcinoma:HT-29, LoVo, SW480, Caco-2, Colon cancer derived xenograph tunr growth, GST pull-down assay Luciferase reporter gene assay Yeast two-hybrid screening Immunofluorescence staining In vitro dephosphorylation assay Flow cytometry Immunoprecipitation and immunoblot analysis Sphere growth assay Soft agar colony formation, microscope and analyzed with ImageJ digital imaging software (Rasband, W.S., ImageJ, U.S. National Institute of Health, Bethesda, MD, USA, http://imagej.nih.gov/ij/, 1997-2014)., Imaris® software Colon adenocarcinoma CD133 molecular marker for cancer stem cells AKT activation, transfection with lentivirus, MS-9096U
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AKIMOTO, Miho, et al. An inhibitor of HIF-α subunit expression suppresses hypoxia-induced dedifferentiation of human NSCLC into cancer stem cell-like cells. World, 2013, 3.4: 41-54.
Human lung adenocarcinoma cells :A549, PC3, PC9, PC14, Small cell carcinoma cells(=Small cell lung cancer): PC6 and QG90 Squamous cell carcinoma cells:PC1, PC10, QG56 Bronchoalveolar carcinoma cells : H358,, Non-small cell lung cancer, Immunocytochemistry Colony formation assay Western blotting analyses, confocal laser microscope, Human lung adenocarcinoma NSCLC Small cell carcinoma(=Small cell lung cancer) Squamous cell carcinoma Bronchoalveolar carcinoma Tumor microenvironment Hypoxia Hypoxia-inducible factor Differentiation Cancer stem cells Hypoxia-inducible factor inhibitor, TX 402 (HIF expression inhibitor) , MS-9035XZ
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BRESLIN, Susan and O’DRISCOLL, Lorraine Three-dimensional cell culture: the missing link in drug discovery. Drug Discovery Today, 2013, 18. 5: 240-249.
http://www.sciencedirect.com/science/article/pii/S1359644612003376
Breast cancer cell lines: MCF7, T-47D, MDA-MB-435, MCF7-ADR,MDA-MB-231, MDA-MB-468 ., 3D Cell Culture Review
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GOUDARZI, Houman, et al. Hypoxia affects in vitro growth of newly established cell lines from patients with malignant pleural mesothelioma. Biomedical Research, 2013, 34.1: 13-21.
https://www.jstage.jst.go.jp/article/biomedres/34/1/34_13/_pdf
Pleural mesothelioma Novel MPM cell lines developed from patients, Pleural mesothelioma, anchorage independent assay, phase contrast microscope ECLIPSE TE300 Nikon scanning electron microscope SEM S-4500 Hitachi, Pleural mesothelioma Hypoxia anchorage independent assay normoxia, hypoxia agent, MS-9096U
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ISHII, Genichiro, et al. Morphophenotype of floating colonies derived from a single cancer cell has a critical impact on tumor‐forming activity. Pathology international, 2013, 63.1: 29-36.
http://onlinelibrary.wiley.com/doi/10.1111/pin.12018/abstract
Human lung adenocarcinoma cell line: A549, Lung adenocarcinoma, Lung adenocarcinoma Epithelial-Mesenchymal Transition (EMT) Floating colony Morphophenotype Single cancer cell Tumor forming activity, MS-9096U
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KATO, Takuma; TANAKA, Masakazu; OBA, Makoto. Protein Transfection Study Using Multicellular Tumor Spheroids of Human Hepatoma Huh-7 Cells. PloS one, 2013, 8.12: e82876.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3857816/pdf/pone.0082876.pdf
Human Hepatoma cell line: Huh-7, Hepatoma, Cellular uptake assay endocytosis assay, CLMS Confocal laser scanning microscope -Carl Zeiss 40X and 100X and TurboFect, Hepatoma DDS drug delivery system transfection Pro-DeliverIN, Xfect, and TurboFect Hoechst UV laser BSA complex, Pro-DeliverIN, Xfect and TuboFect endocytosis inhibitors- sucrose, amiloride,filipin, MS-9096U
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KESSEL, Sarah, et al. (2013). Progressing 3D Spheroid Analysis into a HTS Drug Discovery Method. Molecular biology of the cell, AMER SOC CELL BIOLOGY 8120 WOODMONT AVE, STE 750, BETHESDA, MD 20814-2755 USA.
[Human malignant brainblioma: U87MG, Multidrug, 7AAG, Paclitaxel, Temozolomide (TMZ), Doxorubicin]
Human malignant brainblioma: U87MG, Brainblioma, Fluorecent viability assay Sphere diameter assay, Celigo® Imaging Cytometer, Multidrug, 7AAG Paclitaxel Temozolomide (TMZ) Doxorubicin, MS-9384U
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MIKHAIL, Andrew S.; EETEZADI, Sina; ALLEN, Christine. Multicellular tumor spheroids for evaluation of cytotoxicity and tumor growth inhibitory effects of nanomedicines in vitro: A comparison of docetaxel-loaded block copolymer micelles and Taxotere®. PloS one, 2013, 8.4: e62630.
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0062630
Human cervical cancer cells: HeLa Human colon cancer cell: HT29, Nanomedicines, Tumor spheroid growth and inhibtion of MCTS acid phosphatase (APH) assay-cytotoxic assay, light microscope with a 10X (VWR VistaVision TM) connected to camera (VWR DV-2B)., MCTS survival factor colony forming, BCM+DTX and TaxotereH, MS-9096U
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SATO, Shuji, et al. Identification of the cancer cell proliferation and survival functions of proHB-EGF by using an anti-HB-EGF antibody. PloS one, 2013, 8.1: e54509.
http://dx.doi.org/10.1371%2Fjournal.pone.0054509
Stomach cancer cells: NUGC-3 Bladder cancer cells: 5637 Pancreatic cancer cells: BxPC-3 Ovarian cancer cells: EFO-27, Stomach cancer, Bladder cancer, Pancreatic cancer, Ovarian cancer, Cell Proliferation assay Caspase assay, Cell Titer Glo Caspase-Glo 3/7, Stomach cancer Bladder cancer Pancreatic cancer Ovarian cancerproHB-EGF, MS-9096U
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KONISHI, Hiroaki, et al. PEGylated liposome IHL-305 markedly improved the survival of ovarian cancer peritoneal metastasis in mouse. BMC cancer, 2012, 12.1: 462.
http://www.biomedcentral.com/content/pdf/1471-2407-12-462.pdf
Human ovarian cancer cell line: ES-2, Ovarian cancer, Cytotoxicity of anti-cancer agents MTS assay Immunoblot analysis of EGFR pathways, Cell Titer 96 Aqueous One Solution Proliferation Assay Promega, Ovarian cancer Ascites fluid Drug delivery system (DDS) Peritoneal metastasis, CPT-11 IHL-305 an irinotecan-encapsulated liposome PTX CDDP cisplatin paclitaxel SN38 PD153035, CDDP, PTX,, CPT-11, MS-9096U
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MASUDA, Taisuke, et al. A microfabricated platform to form three-dimensional toroidal multicellular aggregate. Biomedical microdevices, 2012, 14. 6: 1085-1093.
http://link.springer.com/article/10.1007%2Fs10544-012-9713-0
Multicelluar aggregate Three-dimensional Spheroid Chondrocyte Microfabirication, MS-9096U
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NISHIMURA, S, et al. MRGD, a MAS-related G-protein coupled receptor, promotes tumorigenisis and is highly expressed in lung cancer. PLoS ONE, 2012, 7.6: e38618.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3370999/pdf/pone.0038618.pdf
NIH-3T3, 293-10A1, Lung cancer, Cell proliferation of spheroid measured by [3H]-Thymidine incorporation., ATP assay of the speroid, Lung cancer MAS-related G-protein coupled receptor(MRGD), MS-9096U
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OYANAGI, Jun, et al. Epithelial-mesenchymal transition stimulates human cancer cells to extend microtubule-based invasive protrusions and suppresses cell growth in collagen gel. PloS one, 2012, 7.12: e53209.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3534040/pdf/pone.0053209.pdf
Human gastric cancer cells MKN-1 Human lung adenocarcinoma cell line A549 Human pancreatic cancer cell line Panc-1, Gastric cancer Lung adenocarcinoma Pancreatic cancer, EMT(Epithelial-mesenchymal transition ) Immunofluorescent staining Cell migration assays Cell growth assays, Gastric cancer Lung adenocarcinoma Pancreatic cancer EMT(Epithelial-mesenchymal transition ) 24-well Celltight X suspension culture plates, TGF-ß, MS-9024X
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UNO, Makiko, et al. Identification of physiologically active substances as novel ligands for MRGPRD. BioMed Research International, 2012, 2012. 1-9
http://www.ncbi.nlm.nih.gov/pubmed/23091359
HEK293 cells, CHO/dhfr− cells, NIH/3T3, NIH/3T3-RasV12 Dorsal root ganglia (DRG), Endogenous Signal Assay Guanosine 5 -O-(3-Thiotriphosphate) Binding Assay Calcium Flux Assay IP Assay, CellTiter-Glo Luminescent Cell Viability Assay (Promega ARVO SX 1420 Multilabel Counter (Wallac)., Transfection drug screening fibroblast Aminoisobutyric Acids Diethylstilbestrol Mas-related G-protein coupled receptor member D (MRGPRD) G protein-coupled receptor (GPCR) Metabolism Spheroids beta-Alanine, MU6840 MRGPRD antagonists, MS-9096U
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KOSHIKAWA, Naohiko, et al. Proteolytic activation of heparin‐binding EGF‐like growth factor by membrane‐type matrix metalloproteinase‐1 in ovarian carcinoma cells. Cancer science, 2011, 102.1: 111-116.
http://onlinelibrary.wiley.com/store/10.1111/j.1349-7006.2010.01748.x/asset/j.1349-7006.2010.01748.x.pdf?v=1&t=ijwjbloy&s=e098198f045df054dc87d463d702542a0108cac9
Human ovarian carcinoma:cell line: OVCAR-8 and SKOV-3, Ovarian cancer:Ovarian cancer: OVCAR-8, SKOV-3, Counting of live cells hematocytomer by phase contrast microscopy, phase-contrast microscopy (Olympus,, heparin-binding EGF-like growth factor (HB-EGF) membrane-type matrix metalloproteinase-1 (MT1MMP), MT1-MMP inhibitors, MS-9096U
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KUNITA, Akiko, et al. Podoplanin is regulated by AP-1 and promotes platelet aggregation and cell migration in osteosarcoma. The American journal of pathology, 2011, 179.2: 1041-1049.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3157255/pdf/main.pdf
Mouse osteosarcoma cell line: P1.7, P1.9, and P1.15, Dunn MC3T3-E1 mouse osteoblastic cell subclone, AT9.2 Human OS cell lines:MG-63, HOS, and U-2 osteosarcoma, Osteosarcoma, Immunohistochemistry and in Situ Hybridization Semiquantitative RT-PCR and Quantitative Real-Time PCR Platelet aggregation assays Cell migration assay RNA interference Western blot analysis, In Vitro Cell Proliferation Assay, Osteosarcoma Sumilon Celltight Spheroid, MS-9096U
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SAKAMOTO, Hiroshi, et al. CH5424802, a selective ALK inhibitor capable of blocking the resistant gatekeeper mutant. Cancer cell, 2011, 19.5: 679-690.
http://www.sciencedirect.com/science/article/pii/S1535610811001486
Human nonsmall cell lung cancer (NSCLC): NCI-H2228, Non-small cell lung cancer, Viability test: CellTiter-Glo Luminescent Cell Viability Assay (Promega). Caspase-3/7 assay was evaluated using the Caspase-Glo 3/7 Assay Kit (Promega)., luminescence assay and apoptosis assay, Non-small cell lung cancer, CH5424802, ALK kinase inhibitor and Gefitinif, EGFR kinase inhibitor, MS-9096U
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SAKUMA, Yuji, et al. WZ4002, a third-generation EGFR inhibitor, can overcome anoikis resistance in EGFR-mutant lung adenocarcinomas more efficiently than Src inhibitors. Laboratory Investigation, 2011, 92.3: 371-383.
http://www.nature.com/labinvest/journal/v92/n3/pdf/labinvest2011187a.pdf
Human lung adenocarcinoma cell lines HCC827 (E746-A750 deletion) and H1975 (L858RþT790M), Human lung adenocarcinoma, Cell apoptosis assay (Caspase-Glo 3/7 Assay) Western blotting Immunohistochemistry Mutational Analysis, LSM 510 confocal microscope (Carl Zeiss, Human lung adenocarcinoma Anoikis resistance EGFR Lung adenocarcinoma Src（Proto-oncogene tyrosine-protein kinase）, trichostatin A (TSA) ABT-263 AZD0530 (saracatinib) PP1Bcl-2 inhibitor ABT-263 HDAC inhibitor gefitinib WZ4002, MS-9096U
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SHIMIZU, Yutaka, et al. Dienogest, a synthetic progestin, inhibits prostaglandin E2 production and aromatase expression by human endometrial epithelial cells in a spheroid culture system. Steroids, 2011, 76.1: 60-67.
http://www.sciencedirect.com/science/article/pii/S0039128X1000231X
Endometrioma, Endometrioma Dienogest; Prostaglandin E2 Cyclooxygenase-2 Microsomal prostaglandin E2 synthase-1 Aromatase, MS-9096U
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KOGASHIWA, Yasunao, et al. Docetaxel suppresses invasiveness of head and neck cancer cells in vitro. Cancer science, 2010, 101.6: 1382-1386.
http://onlinelibrary.wiley.com/store/10.1111/j.1349-7006.2010.01540.x/asset/j.1349-7006.2010.01540.x.pdf?v=1&t=ijwjds8i&s=d1e73f1dc91029b8cc066aa73a8919d4ec8d2407
Human head cell line: HEp-2 Human neck cell line: SCC Ca9-22, Head and neck cancer, invasiveness of multicellular tumor spheroids, Proliferation Assay (MTT assay) Fujifilm LAS-4000 mini, Squamous cell carcinoma (SCC) of the head and neck Head and neck cancer Polypharmacy tumor cell invasiveness, Docetaxe Cisplatin Fluorouracil, MS-9096U
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MATSUYAMA, Masahiro, et al. Reduced CD73 expression and its association with altered purine nucleotide metabolism in colorectal cancer cells robustly causing liver metastases. Oncology letters, 2010, 1.3: 431-436.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3436221/pdf/OL-01-03-0431.pdf
Human colorectal cancer cell line: SW48 and SW48LM2, Colorectal cancer cells, Viable cell counting, Cell Counting Kit-8 (Dojindo Laboratories, Liver metastases of colon cancer Reduced CD73 expression Purine nucleotide metabolism morphological differences between spheroids of invasive and non-invasive, MS-9096U
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YAMAGUCHI, Shigeru, et al. Novel Photodynamic Therapy Using Water‐dispersed TiO2–Polyethylene Glycol Compound: Evaluation of Antitumor Effect on Glioma Cells and Spheroids In Vitro. Photochemistry and photobiology, 2010, 86.4: 964-971.
http://onlinelibrary.wiley.com/doi/10.1111/j.1751-1097.2010.00742.x/abstract
Rat glioma cell: C6, Glioma, Glioma
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HAN, M, et al. Enhanced percolation and gene expression in tumor hypoxia by PEGylated polyplex micelles. Mol Ther, 2009, 17.8: 1404-1410.
http://www.ncbi.nlm.nih.gov/pubmed/19471245
Human hepatoma HuH-7 cells Human Pancreatic adenocarcinoma BxPC3 cells, Nanomedicine (micelle), Transfection to solid tumors Live/dead assay, Quantitative analysis of uorescent images. The pixels of uorescent area of Cy3-labeled pDNA or transfected Venus protein in the tumor section were quanti ed by using the Image J so ware (http://rsb.info.nih.gov/ij/), and classi ed into three di erent regions by the distance from the injection point (<100 μm, 100–200 μm, >200 μm)., MCTS, multicellular tumor spheroid Cell Hypoxia Cell Line, Tumor Female Gene Expression Regulation, Neoplastic Genetic Therapy/*methods Genetic Vectors/chemistry/*metabolism Humans Mice, Inbred BALB C Mice, Nude *Micelles Molecular Structure Polyethylene Glycols/chemistry Spheroids, Cellular/*metabolism Transfection/methods, DET(N,N-diethyltryptamine), MS-9096U
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WATANABE, Y., et al. A novel translational approach for human malignant pleural mesothelioma: heparanase-assisted dual virotherapy. Oncogene, 2009, 29.8: 1145-1154.
http://www.nature.com/onc/journal/v29/n8/abs/onc2009415a.html
Pleural mesothelioma- human mesothelioma cell lines H2052, H2452, H28 and 211H. Also fibroblast cell line WI38 (JCRB0518), Pleural mesothelioma, Cell Viability assay-XTT assay, Cell profiliration assay II (Roche), Laser-scanning confocal fluorescent microscope GFP expression, laser-scanning confocal fluorescent microscope (Carl Zeiss, Jena, Germany), Pleural mesothelioma MPM ECM extracellular matrix, Adenovirus replication for H2452, showing replciation of virus by detection of GFP expression, MS-9096U
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HAN, Muri, et al. Transfection study using multicellular tumor spheroids for screening non-viral polymeric gene vectors with low cytotoxicity and high transfection efficiencies. Journal of Controlled Release, 2007, 121.1: 38-48.
http://www.sciencedirect.com/science/article/pii/S0168365907002386
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