Transfection

ScreenFect™mRNA is a transfection reagent consisting of a new cationic liposome by click chemistry screening system^*1. ScreenFect™mRNA is suitable for transfection into eukaryotic cells (HeLa, HEK293, MDF cells, etc.) without the replacement of culture medium after transfection because it is not included hazard compounds. ScreenFect™mRNA is used for mRNA transfection procedures.

*1: Biomaterials, Nov.; 33(32): 8160-76, 2012.

ScreenFect™siRNA is a transfection reagent for siRNA consisting of a new cationic liposome screened^*1 by click chemistry. ScreenFect™siRNA is compatible with serum and the transfection operation can be carried out simply because of its low cytotoxicity. The constituent reagents contain no poisonous or deleterious substances.

*1 Biomaterials. 2012 Nov; 33(32):8160-6.

ScreenFect™A is a transfection reagent consisting of a new cationic liposome screened*1 by click chemistry. It can be used with various eukaryote-derived cells and can be added directly to mediums containing antibiotics or serum. DNA and siRNA can be transfected into general experimental cell strains (HeLa, HepG2, MDCK, Cos-7, etc.), stem cells (mouse ES cells, etc.), blood cells (macrophages, THP-1, RAW264, 7, etc.), microglia, primary (initial subculture) cells, and Insect cell. Medium replacement after transfection is not required due to low cytotoxicity. The constituent reagents do not contain any poisonous or deleterious substance.

• High transfection efficiency and low cytotoxicity
• Can be used for both DNA and siRNA
• No need to exchange medium and can be used in the presence of serum