Phos-tag SDS-PAGE can be performed to separate phosphorylated and non-phosphorylated proteins by mixing Phos-tagAcrylamide with acrylamide solution to allow for polymerization to occur.00899_img01.jpg


  • It can be used regardless of the type and position of amino acid residues.
    Able to analyze unknown phosphoproteins.
    Able to detect new phosphorylation sites for which anti-phospho antibodies are not commercially available.
  • Phosphorylated forms with different number and location of phosphorylated sites can be separated.
    Able to determine the level of phosphorylation and the number of phosphorylated forms.
  • It can be used to detect phosphorylated and non-phosphorylated forms simultaneously.
    Able to quantify each phosphorylated form.
    Able to determine the presence of phosphorylation easily.
  • Radioisotopes and special equipment are not needed.
    Experiments can be carried out easily at low costs (Only SDS-PAGE reagents and equipment are required).
  • After electrophoresis, WB- and MS-based analyses and 2D gel electrophoresis can be performed.
    WB: Internal proteins can be analyzed.
    MS: The combinations of phosphorylated sites for each phosphorylated form can be determined.
    2D gel electrophoresis: Phosphorylated forms with identical isoelectric points or molecular weights can be separated.

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