This product is a serum-free liquid media suitable for maintaining human pluripotent stem cells (hPSCs), human ES cells and human iPS cels in the absence of feeder cells. The raw material of this media is not used components containing any animal-derived materials. Moreover, this media does not contain any albumins (such as BSA or HSA), thereby it can culture hPSCs stably without lot difference. Please note that this medium does not contain basic fibroblast growth factor (bFGF).
This product does not contain basic fibroblast growth factor (bFGF).
Store frozen at –20°C. After thawed, store at 2-10°C for up to one week. Do not refreeze.
Human iPS cells 201B7 strain were first cultured in BSA-containing, feeder-free media manufactured by Competitor A. Subsequently, these cells (passage 0) were transferred to StemSure® hPSC MediumΔ (passage 1) and cultured successively. During subculture, we analyzed their morphology, population doubling levels, and undifferentiated state. The results are shown below.
Cultured in StemSure® hPSC MediumΔ (passage 5) and checked expression of the undifferentiated markers (Nanog, Oct3/4, Tra-1-60, SSEA-4, and BC2LCN).
※BC2LCN is a recombinant lectin with high affinity for glycans that exists on the surface of hPSCs.
<Data provided by Dr. Yasuko Onuma and Dr. Yuzuru Ito of the Research Center for Stem Cell Engineering at the National Institute of Advanced Industrial Science and Technology.>
Human ES Cells WA01 strain were first cultured in BSA-containing, feeder-free media manufactured by Competitor A. Subsequently, these cells (passage 0) were transferred to StemSure hPSC Medium (passage 1) and cultured successively. During subculture, we analyzed their morphology, population doubling levels, and undifferentiated state. The results are shown below.
Cultured in StemSure® hPSC MediumΔ (passage 6) and checked expression of the undifferentiated markers(Nanog, Oct3/4, Tra-1-60, SSEA-4, and BC2LCN).
< Data provided by Dr. Yasuko Onuma and Dr. Yuzuru Ito of the Research Center for Stem Cell Engineering at the National Institute of Advanced Industrial Science and Technology. >
Karyotype analysis revealed no chromosomal abnormalities in human iPS cells passaged 27 times in StemSure® hPSC MediumΔ.
< Data provided by Dr. Takumi Miura and Dr, Hidenori Akutsu of the Center for Regenerative Medicine at the National Research Institute for Child Health and Development. >
< Media Composition >
StemSure® D-MEM + StemSure® Serum
Replacement + 2mmol/l L-Glutamine +
0.1mmol/l StemSure® 2-Mercaptoetanol + 1
x Non-essential Amino Acids Solution