SARS-CoV-2 Spike:ACE2 Inhibitor Screening Assay Kit

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  • Product Code: #79931
  • Packaging: 96 rxns.
  • Base price 10200 元
    Sales price 10200 元
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    Description
    Description

    The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Spike protein receptor binding domain (RBD) recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and ACE2 may offer some protection against the viral infection.

    The SARS-CoV-2 Spike:ACE2 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this interaction. The key to this kit is the high sensitivity of detection of bound His-labeled ACE2 by HRP-labeled Anti-His. Only a few simple steps on a microtiter plate are required for the assay. First, SARS-CoV-2 Spike is coated on a 96-well plate. Next, ACE2-His is incubated with SARS-CoV-2 Spike on the plate. Finally, the plate is treated with Anti-His-HRP followed by addition of an HRP substrate to produce chemiluminescence, which then can be measured using a chemiluminescence reader.

    Materials Required But Not Supplied

    PBS (Phosphate buffered saline)
    Luminometer or fluorescent microplate reader capable of reading chemiluminescence
    Adjustable micropipettor and sterile tips

     
    Applications

    This kit is useful for screening inhibitors of SARS-CoV-2 Spike binding to ACE2.

     
    Supplied As
    This kit comes in a convenient 96-well format, with His-labeled ACE2, purified SARS-CoV-2 Spike protein, HRP-labeled anti-His antibody, and assay buffer for 100 binding reactions.
     
    Storage/Stability

    Up to 6 months from date of receipt, when stored as recommended.

     
    Synonym(s)
    ACEH, covid, covid-19, covid19, coronavirus
     
    Format
    Catalog
    Number

    Component

    Amount

    Storage
    79932 SARS-CoV-2 Spike Protein (RBD), mFc Tag 5 μg -80°C

    Avoid
    multiple
    freeze/
    thaw
    cycles!
    11003 ACE2, His-Tag 5 μg -80°C
    79311 3x Immuno Buffer 1 50 ml -20°C
    79728 Blocking Buffer 2 50 ml +4°C
      Anti-His-HRP 15 μl -80°C
    79670 ELISA ECL substrate A (transparent bottle) 6 ml Room
    Temp
    ELISA ECL substrate B (brown bottle) 6 ml Room
    Temp
    79699 96-well white microplate 1 +4°C
     
    Instructions for Use

    See assay kit protocol for detailed instructions.

     
    References

    Hoffmann, M. et al. 2020. Cell181:1-10
    Yan, R. et alScience, In Press 4 March 2020. DOI: 10.1126/science.abb2762

     
    Warnings

    Avoid freeze/thaw cycles.

     
    Scientific Category
    Infectious Disease

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