Product Specifications
CLONE: MID 15B4
HOST: Mouse
ISOTYPE: IgM
IMMUNOGEN: Ceramide (sphingosine-[trans-D-erythro-2-amino-4-octadecene-1,3-diol]) conjugated to BSA.
SOURCE: Purified from ascites by gel filtration on sephacryl S-300.
SPECIES REACTIVITY: Species independent
SPECIFICITY: Recognizes C16- and C24-ceramide, dihydroceramide, sphingomyelin and phosphatidylcholine in highly
artificial lipid overlay test systems. Under more physiological in vitro and in vivo conditions highly specific
for ceramide and does not cross-react with sphingomyelin, cholesterol or other phospholipids.
APPLICATIONS: ELISA, Flow Cytometry, ICC, IHC (PS)
RECOMMENDED
DILUTIONS/CONDITIONS:
ELISA (1:10)
Immunohistochemistry (1:10)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
QUANTITY: 1ml (50 tests). Suggested amount: 20μl/test.
FORMULATION: Liquid. In PBS, pH 7.2, containing 0.5M sodium chloride, 0.1% BSA and 0.09% sodium azide.
SHIPPING: Shipped on Blue Ice
LONG TERM STORAGE: +4°C
REGULATORY STATUS: RUO - Research Use Only
Product Literature References
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Estrogen Metabolite 16a-Hydroxyestrone Exacerbates Bone Morphogenetic Protein Receptor Type II-Associated Pulmonary Arterial Hypertension
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Stimulation of Suicidal Erythrocyte Death by the CDC25 Inhibitor NSC-95397 M. Jemaa, et al. Cell. Physiol. Biochem. 40 597 (2016)
Targeting acid sphingomyelinase with anti-angiogenic chemotherapy J. Jacobi, et al. Cell Signal. 29 52 (2016)
Triggering of Erythrocyte Cell Membrane Scrambling by Emodin M. Mischitelli, et al. Cell. Physiol. Biochem. 40 91 (2016)
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Triggering of Suicidal Erythrocyte Death by Pazopanib E. Signoretto, et al. Cell Physiol. Biochem. 38 926 (2016)
C6-pyridinium ceramide sensitizes SCC17B human head and neck squamous cell carcinoma cells to photodynamic therapy N.B. Boppana, et al. J.
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Enhanced killing of SCC17B human head and neck squamous cell carcinoma cells after photodynamic therapy plus fenretinide via the de novo
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Enhanced Suicidal Erythrocyte Death Contributing to Anemia in the Elderly A. Lupescu, et al. Cell. Physiol. Biochem. 36 773 (2015)
Lipid-induced NOX2 activation inhibits autophagic flux by impairing lysosomal enzyme activity B. Jaishy, et al. J. Lipid Res. 56 546 (2015)
Stimulation of Eryptosis by Narasin G. Bouguerra, et al. Cell Physiol Biochem. 37 1807 (2015)
Ultrasound-stimulated microbubble enhancement of radiation treatments: endothelial cell function and mechanism A.A. Al-Mahrouki, et al.
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Defective macroautophagic turnover of brain lipids in the TgCRND8 Alzheimer mouse model: prevention by correcting lysosomal proteolytic deficits
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Quantitative Ultrasound Characterization of Tumor Cell Death: Ultrasound-Stimulated Microbubbles for Radiation Enhancement H.C. Kim, et al. PLoS
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Effects of biophysical parameters in enhancing radiation responses of prostate tumors with ultrasound-stimulated microbubbles H.C. Kim, et al.
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Aberrant upregulation of astroglial ceramide potentiates oligodendrocyte injury S. Kim, et al. Brain Pathol. 22 41 (2012)
Ceramide mediates nanovesicle shedding and cell death in response to phosphatidylinositol ether lipid analogs and perifosine J.J. Gills, et al. Cell
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Monitoring ceramide and sphingosine-1-phosphate levels in cancer cels and macrophages from tumours treated by photodynamic therapy M.
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Inhibition of SREBP1 sensitizes cells to death ligands Y. Eberhard, et al. Oncotarget 2 186 (2011)
MDR1 (multidrug resistence 1) can regulate GCS (glucosylceramide synthase) in breast cancer cells X. Zhang, et al. J. Surg. Oncol. 104 466 (2011)
Triggering of erythrocyte cell membrane scrambling by ursolic acid K. Jilani, et al. J. Nat. Prod. 74 2181 (2011)
Induction of membrane ceramides: a novel strategy to interfere with T lymphocyte cytoskeletal reorganisation in viral immunosuppression E. Gassert,
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Caspase-dependent and -independent activation of acid sphingomyelinase signaling J.A. Rotolo, et al. J. Biol. Chem. 280 26425 (2005)
Inhibition of erythrocyte phosphatidylserine exposure by urea and Cl K.S. Lang, et al. Am. J. Physiol. Lung Cell Mol. Physiol. 286 F1046 (2004)
Involvement of ceramide in hyperosmotic shock-induced death of erythrocytes K.S. Lang, et al. Cell Death Differ. 11 231 (2004)
PAF-mediated pulmonary edema: a new role for acid sphingomyelinase and ceramide R. Goggel, et al. Nat. Med 10 155 (2004)
Study on ceramide expression and DNA content in patients with healthy mucosa, leukoplakia, and carcinoma of the larynx F.L. Chi, et al. Arch.
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Ceramide inhibits the potassium channel Kv1.3 by the formation of membrane platforms J. Bock, et al. BBRC 305 890 (2003)
Host defense against Pseudomonas aeruginosa requires ceramide-rich membrane rafts H. Grassme, et al. Nat. Med. 9 322 (2003)
Regulation of cell death in mitotic neural progenitor cells by asymmetric distribution of prostate apoptosis response 4 (PAR-4) and simultaneous
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Ceramide-Rich Membrane Rafts Mediate CD40 Clustering H. Grassme, et al. J. Immunol. 168 298 (2002)
Clustering of CD40 ligand is required to form a functional contact with CD40 H. Grassme, et al. J. Biol. Chem. 277 30289 (2002)
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Revised 06-May-20