Phos-tag Tip

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  • Product Code: #387-07321
  • Packaging: 8pcs×10
  • Base price 23900 元
    Sales price 23900 元
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    Phos-Tag Tips 2
    Description

    Phos-tag Agarose Resins in Tip

    Phos-tag Tip is a ready-to-use pipette-tip column prepacked with Phos-tag agarose. This column is a powerful tool for pretreating phosphorylated peptides.
    The Phos-tag molecules in a pipette tip can specifically capture phosphorylated proteins in your samples. This allows for the easy purification of these proteins using only a pipetting operation.

    Features

    • Short hands-on time (less than 30 min)
    • High recovery yields
    • No need for expensive equipment

    Product Information

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    Instructions for Use

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    Application Data

    Separation of Phosphopeptides from the Tryptic Digest of 6 nM β-Casein

    Methods

    • Following trypsin digestion of β-casein (6 nM), phosphorylated peptides were purified using a Phos-tag Tip column.
    • Aliquots of the unpurified sample, flow-through fraction, and eluate were analyzed by HPLC.

    Results

    We detected only phosphopeptide peaks (P1 and P2) in the eluate. This indicates that Phos-tag Tips can selectively trap and enrich phosphopeptides from the tryptic digest of β-casein.

     

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    P1: FQpSEEQQQTERELQDK
    P2: RELEELDVPGEIVEpSLpSpSpSEESITR

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