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FavorPrep™ Plasmid DNA Extraction Mini Kit (sample size:1~5ml culture cells)
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FavorPrep™ Plasmid DNA Extraction Mini Kit (sample size:1~7ml culture cells)
1. FavorPrep™ Plasmid DNA Extraction Mini Kit (sample size:1~5ml culture cells)
✎Specification
Principle: mini spin column (silica matrix)
Sample size: 1 ~ 5 ml br>
Size of plasmid or construct: 15 kb br>
Operation time: 25 minutes
Typical Yield: 20 ~ 30 µg
Binding capacity: 60 µg/ column
Column applicability: centrifugation and vaccum
Important Notes:
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Store RNase A at -20 °C upon recipit of kit.
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Add 0.5 ml of FAPD1 Buffer to a RNase A tube, Dissolve the RNase A by vortexing. Briefly spin the tube and transfer the total RNase A mixture back to the FAPD1 bottle, mix well by vortexing and store the FAPD1 buffer at 4 °C.
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If precipitates have formed in FAPD2 Buffer, warm the buffer in 37°C waterbath to dissolve precipitates.
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Preparation of W1 Buffer and Wash Buffer by adding 96 ~100% ethanol (not provided) for first use.
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Centrifugation steps are done by a microcentrifuge capable of the speed at 11,000 ~1,8000 x g.
✎Component
✎Brief procedure
✎Store at
◆Store at room temperature (15~ 25 ℃) for 2 year. Except RNAse A Powder, store at -20℃.
2. FavorPrep™ Plasmid DNA Extraction Mini Kit (sample size:1~7ml culture cells)
✎Specification
Principle: mini spin column (silica matrix)
Sample size: 1 ~ 7 ml
Size of plasmid or construct: <15 kb
Operation time: <25 minutes
Typical Yield: 25 ~ 40 µg
Binding capacity: 60 µg/ column
Column applicability: centrifugation and vaccum
Important Notes:
-
Store RNase A at -20 °C upon recipit of kit.
-
Add 0.5 ml of FAPD1 Buffer to a RNase A tube, Dissolve the RNase A by vortexing. Briefly spin the tube and transfer the total RNase A mixture back to the FAPD1 bottle, mix well by vortexing and store the FAPD1 buffer at 4 °C.
-
If precipitates have formed in FAPD2 Buffer, warm the buffer in 37°C waterbath to dissolve precipitates.
-
Preparation of W1 Buffer and Wash Buffer by adding 96 ~100% ethanol (not provided) for first use.
-
Centrifugation steps are done by a microcentrifuge capable of the speed at 11,000 ~1,8000 x g.
✎Component
✎Brief procedure
✎Store at
◆Store at room temperature (15~ 25 ℃) for 2 year. Except RNAse A Powder, store at -20℃.
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