Manufacturer’ Articles

New Liposome developed by click chemistry

ScreenFect™mRNA is a transfection reagent consisting of a new cationic liposome by click chemistry screening system*1. ScreenFect™mRNA is suitable for transfection into eukaryotic cells (HeLa, HEK293, MDF cells, etc.) without the replacement of culture medium after transfection because it is not included hazard compounds. ScreenFect™mRNA is used for mRNA transfection procedures.

*1: Biomaterials, Nov.; 33(32): 8160-76, 2012.

  • Direct transfection of mRNA
  • Long RNA transfection
  • Establish iPS cell line without virus vector

Application data

1.GFP mRNA transfection into HEK293 cells

Transfection conditions of ScreenFect™mRNA
  • ・Cell line: HEK293
  • ・Protocol: 1-Step method (See below), no-medium replacement
  • ・Well format: 96 well plate
  • ・Amount of transfection reagent: 0.3 μL/well
  • ・Amount of RNA: GFP mRNA [TriLink #L-6101] 75 ng/well
  • ・Detection time: 24 hours after the transfection
  • ・Detector: Cytation3 microscope [BioTek]

2. EGFP mRNA transfection into HeLa cell (with SFA P-reagent)

Transfection conditions of ScreenFect™mRNA (SF mRNA), SF mRNA + SFA P-reagent
  • [Cell number] 0.7x105 cells/well
  • [Amount of mRNA] 0.1μg/assay
  • [Ratio] mRNA（μg）：ScreenFect™mRNA reagent（μl）= 1：4
  • [Amount of SFA P-reagent] 0.2μl/well 
    　mRNA（μg）：SFA P-reagent（μl） = 1：2
  • [Well format] 24 well plates
  • [Detection time] 48 hours after the transfection

ScreenFect™mRNA transfection conditions

mRNA transfection (/well) Standard Protocol
Plate size Surface area Medium volume Total volume mRNA-lipid complex Rate of mRNA : Transfection Reagent mRNA / Dilution Buffer Transfection Reagent / Dilution Buffer
96 wells 0.3cm2 100μl 10μl 1:3 50ng / 5μl 0.15μl / 5μl
1:4 0.2μl / 5μl
24 wells 2cm2 500μl 50μl 1:3 250ng / 25μl 0.75μl / 25μl
1:4 1.0μl / 25μl
12 wells 4cm2 1000μl 100μl 1:3 500ng / 50μl 1.5μl / 50μl
1:4 2.0μl / 50μl
6 wells 10cm2 2000μl 250μl 1:3 1250ng / 125μl 3.75μl / 125μl
1:4 5.0μl / 125μl

Outline of ScreenFect™mRNA protocol

images

　The ideal mixing ratio of mRNA and transfection reagent varies depending on the type of cells. 
　We will recommend you to examine several ratios and choose the best one.

ScreenFect™mRNA product information

Now on sales campaign! We will offer special price during the campaign. 
[Term of campaign : November 11, 2016 to February 28, 2017 ] For further information, inquires to our distributors.

Code No. Product name ScreenFect™mRNA Transfection Reagent Dilution Buffer for ScreenFect™mRNA Price (CNY)
293-75901 ScreenFect™mRNAicon 0.2ml 10ml 1,350
299-75903 ScreenFect™mRNAicon 1ml 50ml 5,270
297-75904 ScreenFect™mRNAicon 1ml×5 50ml×5 24,120