Manufacturer’ Articles

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This product, called Cellcampus®, is a preparation of type I collagen derived from the scales of farm-raised tilapia, a tropical fish. Cellcampus® is manufactured by Taki Chemical Co., Ltd. in Japan. 00539_img02.jpg

There are no known viruses transmitted from tilapia to humans. Furthermore, tropical fish collagens show higher denaturation temperatures, as compared with collagens derived from non-tropical fish. Thus, Cellcampus® is perfect for tissue and cell culture applications in the field of regenerative medicine. 00539_img03.jpg
It is well documented that tilapia scale collagen exhibits an excellent fibril-forming ability. Moreover, this collagen can enhance cell proliferation. Therefore, Cellcampus® is also ideal for general cell culture protocols.

Features

  • Safe and Reliable Fish-Derived Product
    There is no known fish viruses infect to humans. Thus, fish collagen is a not human health risk.
  • Highly thermostability
    The source of type I collagen is a farm-raised tropical fish (tilapia). The tropical fish (tilapia) collagen has a high denaturation temperature, making it functionally active at typical cell culture temperatures. (Cellcampus® is available for coating cell culture dish surface.)
  • Outstanding Fibril-Forming Ability (see Ref. 1)

Left：Cellcampus® (Collagen isolated from fish scales) 　　Right：Collagen isolated from pig skin
culture dishes were coated with the indicated reagents and subsequently air-dried at room temperature. These atomic force microscopy images show the surface characteristics of the culture dishes. 
Fish collagen extracted from tilapia scales has an increased ability to reassemble into its physiological form (i.e., a fibrillar structure with systematically arranged collagen molecules). Thus, Cellcampus® allows you to coat culture dish surfaces with native collagen fibrils.

Overview

  • Cellcampus®　AO-03A(Liquid)
    Quality Control Testing Acceptance Criteria
    Appearance Clear and colorless solution
    Collagen concentration (%) 0.30～0.36
    Specific optical rotation (°) -350～-450
    pH 3.0～5.0
    Bioburden Undetectable
    Heavy metals(ppm) ≤ 20 (not exceeding the detection limit)
    Arsenic(ppm) ≤ 2 (not exceeding the detection limit)
  • Cellcampus®　FD-08G(Lyophilized)
    Quality Control Testing Acceptance Criteria
    Appearance White sponges
    Weight(g) 0.80～0.90

Application 1

Improved Cell Proliferation (Ref. 2)

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Growth Curve of HeLa Cells

Cellcampus® is effective in promoting cell growth※ 。

  • A similar effect was observed with the cell lines L929, MC3T3, and Saos-2.
  • Cellcampus® can also facilitate the proliferation of cells cultured in low-serum media

Application 2

Enhanced Cell Differentiation (Ref. 3)

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  • Cellcampus® (Collagen from fish scales)
  • Collagen from pig skin
  • No coating

*P＜0.05, **P＜0.005, ***P＜0.0001

[Cell Culture and Assay Methods]
culture dishes were coated with diluted (1:10) Cellcampus® AQ-03A and subsequently air-dried at room temperature.
Osteoblast differentiation was induced in α-MEM supplemented with dexamethasone, β-glycerophosphate, and L-ascorbic acid.
To monitor early differentiation processes, the activity of the osteoblast marker ALP was measured three days after the initiation of culture.

Alkaline phosphatase (ALP) activities were determined after inducing osteoblast differentiation in human mesenchymal stem cells. This result indicates that Cellcampus® can promote the differentiation of these stem cells※.

  • We also confirmed that Cellcampus® can elevate the expression of the early osteoblastic marker genes BMP2 and OPN

References

  1. J. Tanaka,et al. BioIndustry, 26（8）, 26-32（2009）.
  2. Y. Imaizumi, et al. MaterialsIntegration, Vol.23（2）, 27-31（2010）.
  3. R. Matsumoto, et al. BioIndustry, 28（11）, 22-26（2011）.
Coating